Apollo 100 : The Next Generation in Sanger Sequencing

With Apollo 100 the processing of DNA sequencing reactions is easier than ever before. If your lab is trying to reduce costs and time spent on sample preparation and cleanup, Apollo 100 addresses the problem of sample preparation complexity.

This revolutionary new system utilizes exclusive MOV™ pump technology to integrate sequencing reaction setup and cleanup while automating the process on a small-footprint, liquid handling robot.

Simply place a plate of DNA templates on the robotic deck with your sequencing reagents, set the system to run with the user-friendly Apollo 100 Operating Software, and walk away.

In about three hours, a plate of samples is Ready-to-Inject™ into a commercial capillary electrophoresis system such as the AB 3130xl or AB 3730xl.

Apollo 100 reduces the cost of DNA sequencing by enabling efficient scaled-down sequencing reaction setup and cleanup on microchips. Integrating the use of standard four-color sequencing chemistry, paramagnetic bead technology and MOV-driven microfluidic technology, Apollo 100 takes Sanger-based DNA sequencing to a new level of efficiency.

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Customer Benefits

Reduced costs on standard sequencing chemistry. Microfluidic devices allow down-scaling of processes traditionally carried out in larger volumes. Processes that are currently carried out in 10 – 20 µL can be scaled down to 1 µL or less. Not only does this reduce the amount of costly reagents needed; it also reduces the amount of precious sample needed per process.   Further cost reduction through efficient automated on-chip cleanup. The sample preparation workflow for sequencing applications is traditionally fragmented and labor intensive. The process can require up to $100K of equipment, space, and skilled labor. On-chip processing integrates these processes on a platform that is easily automated.   Less hands-on time, freeing up valuable technicians for more complex processes. During each manual step, errors can be introduced and skilled technicians are in short supply. Process automation is also required to meet heavier schedule demands.

Automated preparation of cycle sequencing reactions at nanoliter scale 1. Pipette samples into microtiter plates and place the plate on robotic deck. 2. Samples and reagents are loaded onto microchips robotically. 3. Sample preparation processes are integrated and automated on the microchip: a. 500 nL of sample is mixed with 500 nL of reagents and moved into a reaction chamber. b. Cycle sequencing on the chip begins. c. MOV pumps move clean-up beads into clean-up area. d. MOV pumps move cycle sequencing mix for on-chip cleanup. e. MOV pumps wash out salts and unincorporated labeled ddNTPS. f. MOV pumps elute purified cycle sequencing products. 4. Robot loads cycle sequencing products into microtiter plate. 5. Samples are ready for injection into commercial capillary electrophoresis systems for analysis.